Cell Culture for Pros: Teach Me in 10 Mini-series

Join us for a dynamic webinar series addressing key challenges and innovations in life science research.

Gain insights into scalable bioprocessing strategies that support the growth of Cell and Gene Therapy, from R&D to clinical manufacturing.

Each session offers practical tools and expert guidance to elevate your lab’s performance, safety, and sustainability.

This webinar addresses the critical challenges of variability in cell-based assays, fundamental to research and drug development. We will explore how oftentimes culture conditions, rather than detection reagents impact assay results. Additionally, participants will learn about common contaminants and their impacts, alongside the implementation of standardized protocols and good cell culture Practice guidelines.

Topics covered:

• Replication crisis
• Environmental control and incubation conditions
• Cell culture contaminants
• Optimal cell passaging
• Genetic drift

Speaker:

Dr. Simone Nüssing, Global Field Application Specialist Eppendorf

Our research demonstrates that aerosols generated during simple pipetting can lead to contamination of follow-up samples. This includes a potential spread of contaminants like Mycoplasma. We explored the extent of this contamination and present a practical solution to prevent it, enabling you to protect your cell cultures and ensure the reliability of your experimental outcomes.

Topics covered:

• Mycoplasma threat to your cells
• Aerosol formation during pipetting
• Mycoplasma detection during pipetting
• Advantages of filter tips in pipetting

Speaker:

Dr. Jana Schmidt; Senior Application Specialist Eppendorf

The Heritage of Plastic – Which Options Do We Have?
Sustainability becomes more and more important. Wherever the topic of sustainability emerges in the scientific community, there are many aspects to consider. Plastic-based laboratory consumables are mandatory for many steps in the lab: Purity, sterility, ease-of-use, the expectations of the user are high. But plastic provides a severe heritage: Limited oil resources and waste load.
Is plastic really that severe? What is the real carbon impact? Which alternatives like biobased material or recycling material are the right way to go? Discover the options for plastic & sustainability in the lab.

Topics covered:

• How to reduce waste in the lab
• Recycling options
• BioBased consumables
• ACT Label and certification

Speaker:

Dr. Jan-Hendrik Bebermeier; Global Marketing Manager Eppendorf

In recent years, Cell and Gene Therapies (CGT) emerged as a revolutionary approach to treat hitherto incurable diseases. However, this revolution comes with its own challenges, such as requirements for large cell numbers, minimized contamination risks, as well as controllable and reproducible culture environments. Learn in this short webinar how Eppendorf can support your efforts in the CGT field with innovative bioprocess solutions and the documentation needed to support your transition from R&D to clinical and commercial manufacturing.

Topics covered:

• Stem cells, viral vectors, immune cells
• Inefficiency of 2D culture for large cell numbers
• Implementation of 3D cell culture
• BioBLU^® Single-Use Bioreactors

Speakers:

Christoph van Eickels; Bioprocess Product Lifeczcle Manager Eppendorf
Dr. Stefan Schlösser; Scientific Communications Manager Eppendorf

Discover how to tailor centrifugation parameters to achieve optimal results without compromising cell viability. We will explore strategies for determining the ideal centrifugation time, selecting appropriate temperatures, and managing acceleration and deceleration rates to minimize cell damage. Learn practical tips on pre-cooling or warming centrifuges efficiently, and gain insights into operating centrifuges at varying temperatures. Whether you're troubleshooting current protocols or seeking new efficiencies, this webinar offers valuable knowledge and adaptable strategies to refine your centrifugation processes.

Topics covered:

• Effects of centrifugation on cells
• Tailoring centrifugation parameters for cell culture
• Optimization approach

Speakers:

Dr. Kerstin Isermann; Global Field Application Specialist Eppendorf
Natascha Weiß; Global Field Application Specialist Eppendorf

Learn how Eppendorf devices, solutions, and expertise can streamline your Cell Culture processes and increase your reproducibility. From thawing to harvesting, Eppendorf provides the tools you need to get the most out of your culture workflows. Discover the benefits of Eppendorf ThermoMixer^® cell thawing, safer pipetting with Eppendorf Conical Tubes, reduced contamination risk with filter tips, improved precision with Multipettes, and easy cleaning/sterilization in CellXpert^® CO2 incubators.

Topics covered:

• Eppendorf ThermoMixer C and SmartBlocks
• Eppendorf Conical Tubes 25 mL
• ep Dualfilter T.I.P.S.^® BioBased
• Multipette^® M4 and E3/E3X with Combitips^® advanced
• CellXpert C170i CO2 Incubator

Speaker:

Dr. Christian Haberlandt; Global Marketing Manager Eppendorf

Pipetting in cell culture can introduce shear stress, significantly impacting cells by altering gene expression, affecting cell growth, differentiation, and viability. If these changes occur unintentionally, they can critically influence experimental outcomes. This webinar aims to provide an overview of various strategies to minimize shear forces during pipetting, ensuring the integrity and viability of cells during liquid handling and improving experimental outcomes in cell culture applications.

• Download App Note 442 to read all about the influence of different pipetting systems on cell death and proliferation.
• Visit https://www.eppendorf.link/simply-better-pipetting to learn more about Eppendorf electronic pipettes

Topics covered:

• What is Shear Force in liquids?
• Shear Forces in Cell Culture
• Strategies to minimize shear stress

Speaker:

Dr. Maren Rudolph; Global Field Application Specialist Eppendorf

Thawing frozen cells is an essential, but sometimes undervalued element in all areas of cell-based research and production. Nowadays, thawing of cells by submerging the cryovial in a water bath is still the most common technique, although the procedure mostly lacks standardization and the risk of introducing a contamination is well known. By the absence of water, the risk of introducing a contamination during the process is reduced to a minimum. The Eppendorf ThermoMixer^® C in combination with the SmartBlock cryo thaw provides a water free and automated method for thawing of cells, which allows highly convenient and reproducible thawing procedures. In this webinar, we show that this method is not only well suited for cell lines, but also for sensitive stem cells.

Topics covered:

• Contamination risks and downsides of other methods
• Thawing of cells with Eppendorf ThermoMixer C
• Viabilities of valuable cell lines

Speaker:

Dr. Steffen Riethmüller; Global Field Application Specialist Eppendorf

Explore the advantages of automating liquid handling in cell culture workflows. We’ll discuss how automation improves sample throughput, reproducibility, and precision, while reducing contamination risks and manual errors. Key topics include best practices for aseptic techniques, the impact of pipetting skills, and the benefits of both semi-automated and fully automated liquid handling systems. Attendees will gain insights into optimizing cell-based experiments, enhancing lab productivity, and maintaining consistent, high-quality results through the use of modern automation platforms.

Topics covered:

• Clean and consistent results of automated liquid handling
• Increased throughput and reproducibility
• Sample security and decreased contamination risks
• Time savings for lab personnel

Speaker:

Dr. Jessica Wagener; Global Field Application Specialist Eppendorf

An overview of key factors that influence mammalian shake cultures, including vessel choice, cultivation conditions, and the influence of the operator. We will learn practical strategies to optimize your shake cultures for improved consistency and cell growth.

Topics covered:

• How vessel selection influences shake cultures
• How orbit and shaking speed contribute to growth
• The effects of chamber atmosphere of viability and reproducibility

Speaker:

Ines Kristina Hartmann, Global Field Application Specialist Eppendorf